Figure 5

The circ_8521/miR-324/LC3A axis regulates SVA infection. PK-15 cells were transfected with LC3A siRNA. NC siRNA served as a control. At 24 h post-transfection, the cells were infected with SVA for 3, 10.5, or 18 h (A, B). A LC3A (left) and SVA VP1 mRNA (right) levels were detected by qRT-PCR. B The expression of LC3A and SVA proteins were examined by Western blotting. C PK-15 cells were transfected with an miR-324 mimic. NC mimic served as a control. At 24 h post-transfection, the cells were infected with SVA for 3, 10.5, or 18 h. SVA VP1 mRNA levels were detected by qRT-PCR (upper panel). SVA protein expression was examined by Western blotting (lower panel). D. PK-15 cells were transfected with an miR-324 inhibitor. NC inhibitor served as a control. At 24 h post-transfection, the cells were infected with SVA for 3, 10.5, or 18 h. SVA VP1 mRNA levels were detected by qRT-PCR (upper panel). SVA protein expression was examined by Western blotting (lower panel). E PK-15 cells were co-transfected with an miR-324 inhibitor and circ_8521 siRNA. At 24 h post-transfection, the cells were infected with SVA for 12 h. SVA VP1 mRNA levels were detected by qRT-PCR (upper panel). SVA protein expression was examined by Western blotting (lower panel). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.