Figure 3
From: circRNA_8521 promotes Senecavirus A infection by sponging miRNA-324 to regulate LC3A
circ_8521 regulated SVA infection in PK-15 cells. PK-15 cells were transfected with circ_8521 siRNA (50 nM) (A–C) or the circ_8521 overexpression plasmid pEZX-circ_8521 (0.5 ng) (D–F). NC siRNA served as a control. At 24 h post-transfection, cells were infected with SVA for 3, 10.5, or 18 h. The effect of circ-8521 on SVA infection was subsequently analyzed. A qRT-PCR analysis circ_8521 levels. B qRT-PCR analysis SVA VP1 mRNA levels. C Western blotting analysis of SVA protein expression. D qRT-PCR analysis of circ_8521 levels. E qRT-PCR analysis SVA VP1 mRNA levels. F Western blotting analysis of SVA protein expression. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.