Figure 2
From: circRNA_8521 promotes Senecavirus A infection by sponging miRNA-324 to regulate LC3A
Identification of circ_8521 as an SVA-inducible host circRNA. A Schematic illustration showing that the genomic sequence of circ_8521 is derived from five exons (spanning 690 nt) of the AGO3 gene. The sequences of the circ_8521 junction site are also shown. B qRT-PCR analysis of GAPDH RNA and circ_8521 levels after RNase R treatment. C The levels of circ_8521 (left) and SVA VP1 mRNA (right) in PK-15 cells at 0, 3, 10.5, 18 h post-infection (hpi) were determined by qRT-PCR. D The levels of circ_8521 were assessed by qRT-PCR in nuclear and cytoplasmic fractions of the PK-15 cell lysate. GAPDH and U6 served as the cytoplasmic and nuclear localization controls, respectively. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.