Figure 3

Screen of 41 compounds for their antiviral activity against WNV in eNPCs. A Schematic representation of the phenotypic screen. Mol., Molecule (B) Screen of 41 compounds at 10 µM. C Screen of 16 compounds at 1 µM. A hit was arbitrarily defined as reducing infection by 25% (below the green dashed line) and showing less than 20% toxicity (above the red dashed line). Results are representative of 2 independent screens (at each dose) performed in quadruplicate. Total number of cells and percentage of infected cells were normalized to control (non-treated WNV-infected eNPCs). Data are expressed as the mean ± SD. Comparison between WNV-infected treated and non-treated cells was performed with a two-tailed unpaired Mann–Whitney test with p-values significant when *p < 0.05, **p < 0.01.