Genomic characterization of equine influenza A subtype H3N8 viruses by long read sequencing and functional analyses of the PB1-F2 virulence factor of A/equine/Paris/1/2018

Table 1 Sequencing statistics

	South Africa	Richmond	Beuvron	Paris
Total reads	515 999	519 851	976 418	582 633
Filtered reads (Q > 10; Size > 600 bp)
Reads number	158 077	173 495	420 018	189 296
Average length (nt)	1291	1132	974	1215
Average quality	22.5	22.6	22.1	22.6
Mapped reads
Total reads mapped	157 979	173 393	419 899	188 939
% reads mapped	99.94%	99.94%	99.97%	99.81%
Segment1 (PB2)	20 197	27 727	80 078	26 715
Segment2 (PB1, PB1-F2)	18 731	26 059	135 601	26 609
Segment3 (PA)	19 554	24 642	31 455	33 794
Segment4 (HA)	17 898	21 245	35 347	28 913
Segment5 (NP)	27 086	16 715	19 096	20 082
Segment6 (NA)	23 350	21 141	29 387	21 860
Segment7 (M1–M2)	26 425	35 844	54 494	35 756
Segment8 (NS1, NEP)	23 634	17 339	47 279	25 569

Detailed sequencing statistics obtained after demultiplexing and size and quality filtering of the sequenced strains A/equine/South Africa/4/2003 (South Africa), A/equine/Richmond/1/2007 (Richmond), A/equine/Beuvron-en-Auge/2/2009 (Beuvron), and A/equine/Paris/1/2018 (Paris).

ISSN: 1297-9716