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Figure 1 | Veterinary Research

Figure 1

From: Genomic characterization of equine influenza A subtype H3N8 viruses by long read sequencing and functional analyses of the PB1-F2 virulence factor of A/equine/Paris/1/2018

Figure 1

Schematic workflow implemented for long-read sequencing of equine influenza virus. The four equine influenza viruses A/equine/Beuvron-en-Auge/2/2009, A/equine/Paris/1/2018, and OIE recommended vaccine strains A/equine/South Africa/4/2003 and A/equine/Richmond/1/2007 were analyzed. A After viral amplification in the MDCK cell line and RNA extraction, the eight genomic segments were individually amplified by RT‒PCR. Amplified DNA products were controlled by capillary electrophoresis. B For each strain, the eight amplicons were pooled with equimolar ratios, and sequencing libraries were prepared and loaded on a flow cell. C The bioinformatics workflow used from raw data to consensus sequence construction. The reference strain is A/equine/Ohio/2005 (GenBank accession numbers: CY067323, CY067324, CY067325, CY067326, CY067327, CY067328, CY067329, CY067330).

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Veterinary Research

ISSN: 1297-9716