Figure 5

Pasteurella multocida activates the Hippo-Yap pathway by inducing Rassf1 expression. A Analysis of Hippo-Yap pathway regulators based on RNA-seq data. Blue boxes indicate significant decreases and red boxes indicate significant increases. B Analysis of Hippo pathway regulators at the mRNA level. The upper part was analyzed by RNA-seq. The lower part was validated by q-PCR results. Each q-PCR group consists of 5 individuals at 16 hpi. C Immunohistochemical staining of Rassf1 in mouse lungs. Mice were infected with 10⁴ CFU PmCQ2 for 16 h. Scale bar = 100 µm. D Western blot analysis of Rassf1 in mouse lungs. Mice were infected with 10⁴ CFU PmCQ2 for 16 h. E Western blot analysis of Rassf1 in TC-1 cells infected with 1 MOI PmCQ2. F Western blot analysis of TC-1 cells based on shRNA knockdown of Rassf1. G Western blot analysis of TC-1 cells infected with 1 MOI PmCQ2 based on shRNA knockdown of Rassf1. H Analysis of apoptosis rates of TC-1 cells infected with 1 MOI PmCQ2 plus shGFP or shRassf1 at 10 hpi. On the right is quantitation of apoptosis positive cells. ***p < 0.001.