Figure 4
From: Pasteurella multocida activates Rassf1-Hippo-Yap pathway to induce pulmonary epithelial apoptosis
Pharmaceutical inhibition of the Hippo-Yap pathway attenuates Pasteurella multocida infection.A Scheme presenting the XMU-MP-1 (XMU) treatment protocol. B H&E staining of the lungs of control, 104 CFU PmCQ2-infected and 104 CFU PmCQ2 plus 3 mg/kg XUM-MP-1 infected mice. Mice were infected for 32 h. Scale bar = 100 µm. The graph below shows the survival rates of control, 104 CFU PmCQ2-infected, and 104 CFU PmCQ2-plus 3 mg/kg XUM-MP-1-infected mice. C TUNEL staining of lungs from control, 104 CFU PmCQ2-infected and 104 CFU PmCQ2 plus 3 mg/kg XUM-MP-1-infected mice. Scale bar = 100 µm. The right panel shows the quantification of TUNEL-positive cells in 3 random sections. D Bacterial load in mice infected with 104 CFU PmCQ2 and mice infected with 104 CFU PmCQ2 plus 3 mg/kg XUM-MP-1. Mice were infected for 16 h. E Quantification of IL-6, TNF-α, and IL-1β in the serum of PmCQ2-infected mice and PmCQ2-infected mice with XMU-MP-1 treatment at 16 hpi by ELISA. F Immunohistochemistry staining of p-Lats1, p-Yap and Yap in mice lungs infected with 104 CFU PmCQ2 group and infected with 104 CFU PmCQ2 plus XUM-MP-1 group. Scale bar = 200 µm. G Western blot analysis of the dynamics of Hippo pathway infection in 104 CFU PmCQ2 group and 104 CFU PmCQ2 plus XUM-MP-1 group mice at 16 hpi. *p < 0.05, **p < 0.01.