Figure 3

Pasteurella multocida activation of the Hippo-Yap pathway causes pulmonary epithelial apoptosis. A Western blot analysis of the dynamics of the Hippo-Yap pathway in mouse lung epithelial cells after TC-1 infection with 1 MOI PmCQ2. B Analysis of Yap expression at the mRNA level in mouse lung epithelial cells 8 h after TC-1 infection with 1 MOI PmCQ2. C Western blot analysis of mouse lung epithelial cells TC-1 infected with 1 MOI PmCQ2 with or without 10 µM proteasome inhibitor MG-132. D Western blot analysis of the dynamics of the Hippo-Yap pathway in mouse lung epithelial cells TC-1 infected with 1 MOI PmCQ2 plus vector or 1 µM XMU-MP-1 for 6 or 8 h. E Western blot analysis of Yap subcellular localization in TC-1 infected with 1 MOI PmCQ2 with or without 1 µM XMU-MP-1 for 8 h. F Cell viability analysis of TC-1 cells infected with 1 MOI PmCQ2 plus vector or different concentrations of XMU-MP-1 for 10 h. Cell viability was estimated by LDH levels in the cell. G Analysis of apoptosis rate of TC-1 cells infected with 1 MOI PmCQ2 plus vector or different concentrations of XMU-MP-1 for 10 h. The right panel shows the quantification of apoptosis-positive cells. H A scheme presents the Transwell assay protocol. At the right are quantifications of translocated bacteria count of TC-1 cells treated with 1 µM XMU-MP-1 or Vehicle for 8 h. Each group contained 3 replicates. **p < 0.01, ***p < 0.001, N.S: no significant.