Figure 2
From: Pasteurella multocida activates Rassf1-Hippo-Yap pathway to induce pulmonary epithelial apoptosis
Pasteurella multocida activates the Hippo-Yap pathway. A On the left, the first 5 RNA-seq-enriched pathways and the first 5 down-regulated signaling pathways of cellular processes in mouse lungs after infection with 104 CFU PmCQ2 at 24 hpi. B Hippo-Yap pathway analysis in RNA-seq. Blue boxes indicate significant down-regulation. C Analysis of the Hippo pathway core components at the mRNA level. The upper part was analyzed by RNA-seq. The lower part was validated by q-PCR. q-PCR was performed on 5 individuals per group at 16 hpi. D Immunohistochemical staining of p-Lats1 and Yap in the lungs of wild-type and mice infected with 104 CFU of PmCQ2 for 16 h. Scale bar = 100 µm. E Western blotting to analyze the dynamics of the Hippo pathway in the lungs of wild-type and mice infected with 104 CFU PmCQ2 for 16 h. *p < 0.05, **p < 0.01, ***p < 0.001, N.S: no significant.