Figure 1
From: Pasteurella multocida activates Rassf1-Hippo-Yap pathway to induce pulmonary epithelial apoptosis
Pasteurella multocida-induced pulmonary epithelial apoptosis is critical for its pathogenesis. A Mice were infected intranasally with 104 CFU PmCQ2 for 32 h. Scale bar = 100 µm. Bottom is the survival curve of mice infected intranasally with 104 CFU PmCQ2. B TUNEL staining of wild-type mouse lungs or 104 CFU PmCQ2 mouse lungs at 16 h. The right panel shows the number of TUNEL-positive cells in 3 random sections. C Immunohistochemical staining of Sp-c in the lungs of wild-type mice and Sp-c in the lungs of mice intranasally infected with 104 CFU PmCQ2 at 16 h post-infection (hpi). The graph on the right shows the number of Sp-c-positive cells in 3 random sections. D Representative images of confocal analysis of Sp-c staining and TUNEL staining in lungs of mice infected in situ with 104 CFU PmCQ2 or saline at 16 hpi. Zoom bar = 50 μm. Red arrows indicate apoptotic lung epithelium. E Bacterial load in the lungs, liver, kidneys and bloodstream of mice infected with 104 CFU PmCQ2 at 16 hpi. F For Ac-DEVD-CHO (CHO) treatment, mice were injected intranasally with 2 mg/kg CHO 30 min before infection. Survival curves of PmCQ2-infected mice with or without CHO treatment. Each group consists of 8 mice. Each point represents one individual. *p < 0.05, ***p < 0.001.