Figure 9

Role of NCP BVDV-induced mitophagy on apoptosis. A MDBK cells were transfected with siParkin or siNC for 24 h. Then, the cells were infected with NCP BVDV (MOI = 5), Western blot assays were performed to determine the CYTC, cleaved-caspase3, cleaved-PARP and NS4B expression at 48 hpi. B MDBK cells were transfected with siParkin or siNC for 24 h. Then, the cells were infected with NCP BVDV (MOI = 5), the activities of caspase-3 were determined at 48 hpi. C, D MDBK cells were transfected with siParkin or siNC for 24 h. Then, the cells were infected with NCP BVDV (MOI = 5), apoptosis was detected by flow cytometry (C) and TUNEL (D) assays at 48 hpi, respectively. Data are given as means ± standard deviation (SD) from three independent experiments. P values were calculated using the Student t test. An asterisk indicates a comparison with the indicated control. *P < 0.05; **P < 0.01; ns: not significant.