Figure 2

Drp-1-mediated mitochondrial fission facilitates the progression of NCP BVDV infection. A Western blot analysis of Drp1 and p-Drp1-S616 expression in mock and NCP BVDV-infected cells at indicated time point post infection. CCCP (10 μM) group was collected at 12 h post-incubation (hpi). B IFA analysis of the recruitment of Drp1 to damaged mitochondria in mock and NCP BVDV-infected (MOI = 5) cells at 48 hpi. (Scale bar = 10 μm). C MDBK cells were transfected with siDrp1 or siNC for 24 h and infected with NCP BVDV (MOI = 5), Mito Tracker Red-labeled mitochondrial morphology was observed by IFA assay. CCCP (10 μM) group was collected at 12 hpi. (Scale bar = 10 μm). The mean mitochondrial length was calculated using Image J software (Version 1.53t) (NIH). D MDBK cells were transfected with siDrp1 or siNC for 24 h. Then, the cells were infected with NCP BVDV (MOI = 5). qRT-PCR (left) and Western blot (right) assays were performed to determine the viral replication and progeny at 24 hpi and 48 hpi. E Viral replication levels in MDBK cells stably expressing Drp1 followed by NCP BVDV (MOI = 5) infection were detected by qRT-PCR (left) and Western blot (right) at 48 hpi. Data are given as means ± standard deviation (SD) from three independent experiments. P values were calculated using the Student t test. An asterisk indicates a comparison with the indicated control. *P < 0.05; **P < 0.01; ns: not significant.