Table 1 Medium compositions.
From: A matter of differentiation: equine enteroids as a model for the in vivo intestinal epithelium
Component | PM | DM1 | DM2 | DM3 | DM4 | Manufacturer; # Catalog Number |
|---|---|---|---|---|---|---|
Basal medium (BM)* |  +  |  +  |  +  |  +  |  +  |  |
B-27™ supplement | 1 × | 1 × | 1 × | 1 × | 1 × | Thermo Fisher Scientific, Waltham, MA, USA; # 17504001 |
N-2 | 1 × | 1 × | 1 × | 1 × | 1 × | Thermo Fisher Scientific, Waltham, MA, USA; # 17502001 |
Nicotinamide | 10 mM | − | − | − | − | Merck, Vienna, Austria; # 72 340 |
n-Acetyl-l-Cysteine | 1 mM | − | − | − | − | Merck, Vienna, Austria; # A9165 |
A 83–01 | 500 nM | 500 nM | 500 nM | − | − | Hycultec GmbH, Beutelsbach, Germany; # HY-10432 |
SB 202190 | 10 µM | 10 µM | 10 µM | − | − | Hycultec GmbH, Beutelsbach, Germany; # HY-10295 |
hGastrin I | 10 nM | − | − | − | − | Hycultec GmbH, Beutelsbach, Germany; # HY-P1097 |
hHGF | 50 ng/mL | − | − | − | − | Thermo Fisher Scientific, Waltham, MA, USA; # 100-39H |
mEGF | 50 ng/mL | 50 ng/mL | 50 ng/mL | 50 ng/mL | 50 ng/mL | Thermo Fisher Scientific, Waltham, MA, USA; # 315–09 |
CHIR-99021 | − | − | 2.5 µM | − | − | BioGems; Westlake Village, CA, USA; # 2 520 691 |
LY2157299 | − | − | 500 nM | − | − | TargetMol®, Wellesley Hills, MA, USA; # T2510 |
hNoggin | 100 ng/mL | 100 ng/mL | 100 ng/mL | 100 ng/mL | − | Thermo Fisher Scientific, Waltham, MA, USA; # 120-10C |
Wnt3A conditioned medium# | 43% v/v | 43% v/v | 43% v/v | − | − | In house manufacturing (Division of Small Animal Internal Medicine, University of Veterinary Medicine Vienna) |
Rspo1 conditioned medium# | 10% v/v | 10% v/v | 10% v/v | 10% v/v | − | In house manufacturing (Division of Small Animal Internal Medicine, University of Veterinary Medicine Vienna) |
Gentamicin | 20 µg/mL | 20 µg/mL | 20 µg/mL | 20 µg/mL | 20 µg/mL | Merck, Vienna, Austria; # G1272 |
DAPT | − | − | − | − | 5 µM | Hycultec GmbH, Beutelsbach, Germany; # HY-13027 |
Y-27632 dihydrochloride | 10 µM | 10 µM | 10 µM | 10 µM | − | TargetMol®, Wellesley Hills, MA, USA; # T1725 |