Figure 5
From: Molecular mechanism of bovine Gasdermin D-mediated pyroptosis
bCaspase-3 cleaved bGSDMD at residue D86. HEK293T cells were individually transfected with indicated plasmids for 24 h. A and B Cell lysates were analyzed with anti-Flag, anti-GFP, and anti-GAPDH by Western blot. C The supernatants were collected and analyzed for LDH release levels. D Microscopic images of the indicated HEK293T cells. Scale bar, 50 μm. * P < 0.05, ** P < 0.01, *** P < 0.001