Figure 4
From: Molecular mechanism of bovine Gasdermin D-mediated pyroptosis
The loop aa 278–299, L293 and A380 are essential to maintain the autoinhibition of bGSDMD. HEK293T cells were individually transfected with indicated plasmids for 24 h. A and C The supernatants were collected and analyzed for LDH release levels. B and D Microscopic images of the indicated HEK293T cells. Scale bar, 50 μm. * P < 0.05, ** P < 0.01, *** P < 0.001