Figure 3
From: Molecular mechanism of bovine Gasdermin D-mediated pyroptosis
T238 and F239 are the key sites for bGSDMD NT to induce pyroptosis. HEK293T cells were individually transfected with indicated plasmids for 24 h. A and C The supernatants were collected and analyzed for LDH release levels. B and D Microscopic images of the indicated HEK293T cells. Scale bar, 50 μm. E and F Cell lysates were immunoprecipitated with anti-Flag antibody and then analyzed with anti-Flag and anti-HA antibodies by Western blot. * P < 0.05, ** P < 0.01, *** P < 0.001