Figure 2
From: Molecular mechanism of bovine Gasdermin D-mediated pyroptosis
Residue D277 of bGSDMD is the cleavage site for bCaspase-1. HEK293T cells were individually transfected with indicated plasmids for 24 h. A The supernatants were collected and analyzed for LDH release levels. B Microscopic images of the indicated HEK293T cells. Scale bar, 50 μm. C Cell lysates were analyzed with anti-Flag, anti-Myc, and anti-GAPDH by Western blot. * P < 0.05, ** P < 0.01, *** P < 0.001