Figure 3
From: Construction of recombinant fluorescent LSDV for high-throughput screening of antiviral drugs
The EGFP stability in LSDV-ΔTK/EGFP during passaging in MDBK cells. A The EGFP expression in MDBK cells after infection with LSDV-ΔTK/EGFP of P1–P20. The expression of EGFP was detected under a fluorescent microscope at 96 hpi. B The RT-PCR detection of the TK gene in MDBK cells after infection with LSDV-ΔTK/EGFP of P1–P20 The resulting RT-PCR products were resolved by 1% agarose gel electrophoresis. C The LSDV-ΔTK/EGFP titer of P1, P5, P10, P15 and P20 generation.