Figure 2From: Construction of recombinant fluorescent LSDV for high-throughput screening of antiviral drugsCharacterization of LSDV-WT and LSDV-ΔTK/EGFP. A The proliferation of recombinant viruses in VERO cells was observed in mock, first generation, and second generation. B The RT-PCR detection of the EGFP gene in MDBK cells infection with LSDV-WT or LSDV-ΔTK/EGFP. C Growth kinetics curves of LSDV-WT and LSDV-ΔTK/EGFP in the MDBK cells at MOI = 1. D The morphology of CPE in MDBK cells of LSDV-ΔTK/EGFP and LSDV-WT on 4 dpi.Back to article page