Figure 6
Pathological findings in brown bear 47 showing distemper. A Head. Subcutaneous and muscular hematomas and fracture of the parietal bone can be observed. B Liver. Petechial hemorrhages (arrows) and edema of the wall of gall bladder (asterisk) are observed in visceral surface. C Liver. Necrosis of hepatocytes and presence of a basophilic intranuclear inclusion body (arrow), without associated inflammatory infiltration, are shown. Hematoxylin and eosin stain. D Lymph node. Many syncytia formation (multinucleated giant cells) located in the cortical region are observed. Phenomena of karyorrhexis and karyolysis in several lymphocytes can be also observed. Hematoxylin and eosin stain. E Brain: cerebellum. Widespread necrosis and depletion of Purkinje cells in two cerebellar folia is shown. Hematoxylin and eosin stain. Inset: Proliferation of Bergmann glial cells is revealed using immunohistochemistry. Avidin biotin complex, Iba1 primary antibody. F Adrenal gland: canine distemper virus (CDV) antigen is detected within endothelial cells of blood capillaries located in medulla using immunohistochemistry. Avidin biotin complex, anti-CDV primary antibody.