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Figure 3 | Veterinary Research

Figure 3

From: Variation in the affinity of three representative avian adenoviruses for the cellular coxsackievirus and adenovirus receptor

Figure 3

Coimmunoprecipitation (co-IP) and colocalization analyses of CAR and the fibre knob. A‒K Cells were co-transfected with pFlag-Fibre and pMyc-CAR using mouse anti-Flag and rabbit anti-Myc mAbs. After 36 h of incubation at 37 °C, immunoblotting analysis was performed to evaluate the binding of knob to different domains of CAR in the immunoprecipitants. L, M Analysis of the cell surface distribution and colocalization of CAR and fibre knob was performed by indirect immunofluorescence assay in CHO cells. The fibre protein (red fluorescence) colocalized with the CAR protein (green fluorescence) in the cytoplasm of 293T cells. The nuclei were labelled with DAPI (blue points).

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Veterinary Research

ISSN: 1297-9716