Figure 2
Effect of resveratrol on antimicrobial compound production in goat mammary epithelial cells (GMECs). β-defensin-1, S100A7, and lactoferrin levels as measured by ELISA in GMECs treated with resveratrol at various concentrations for 3 days (A) or treated with 10 μM resveratrol each day (B). C The expression of BD1 (β-defensin-1), S100A7, and LTF (lactoferrin) mRNA was detected using qPCR in GMECs treated with 10 μM resveratrol for 3 days. D β-defensin-1, S100A7, and lactoferrin levels as measured by ELISA in the medium of the upper chamber of inserts treated with 10 μM resveratrol for 4 days. Control GMECs were treated with 0.1% dimethyl sulfoxide. The data are presented as the mean ± SEM (n = 8–12). Asterisks show significant differences (p < 0.05 versus control).