Figure 1
Effect of resveratrol on the tight junction barrier of goat mammary epithelial cells (GMECs). Western blot analysis of claudin-3 and claudin-4 in GMECs treated with resveratrol at various concentrations for 3 days (A) or treated with 10 μM resveratrol each day (B). The graphs show the results of densitometry analysis; α-tubulin was the internal control. C The images show the localization of claudin-3 (green) and occludin (red; tight junction marker) in GMECs treated with 10 μM resveratrol for 4 days. Scale bar 10 µm. D Western blot analysis of Triton X-100-soluble and -insoluble fractions of claudin-3. The graph shows the ratio of insoluble/soluble claudin-3. E The graph shows transepithelial resistance in GMECs. Control GMECs were treated with 0.1% dimethyl sulfoxide. The data are presented as the mean ± SEM (n = 8–12). Asterisks show significant differences (p < 0.05 versus control).