Molecular basis for the different PCV2 susceptibility of T-lymphoblasts in Landrace and Piétrain pigs

Table 1 Information of antibodies used in this study

Antibody name	Class	Host	Mono/poly-clonal	Dilution for IF	Distributor	Catalog number
Anti-PCV2-Cap	IgG2b	Mouse	Mono	1:50	Made in our lab	–
Anti-PCV2-Rep	IgG	Rabbit	Poly	1:500	GeneTex; Irvine, CA, USA	GTX133638
Anti-CD3	IgG1	Mouse	Mono	1:100	Bio-Rad; Hercules, CA, USA	MCA5951GA
Anti-HS	IgM	Mouse	Mono	1:50	Amsbio; Abingdon, OX, UK	370255
Anti-CS	IgM	Mouse	Mono	1:50	Sigma-Aldrich; St. Louis, MO, USA	C-8035
Anti-decorin	IgG	Rabbit	Poly	1:50	GeneTex; Irvine, CA, USA	GTX101250
Anti-PTPRZ	IgG	Rabbit	Poly	1:50	Abbexa; Cambridge, CB, UK	abx131519
Anti-Rab5	IgG	Rabbit	Poly	1:50	Proteintech; Rosemont, IL, USA	11947-1-AP
Anti-Rab7	IgG	Rabbit	Poly	1:50	Cusabio; Houston, TX, USA	CSB-PA019219LA01HU
Anti-LAMP1	IgG	Rabbit	Poly	2:50	Proteintech; Rosemont, IL, USA	21997-1-AP

All relevant secondary antibodies used in this study were all purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). The incubation of secondary antibodies was performed and optimized according to the instructions of the manufacturers.

ISSN: 1297-9716