Figure 9

Co-localization analysis of PCV2 intracellular trafficking. Confocal microscopy was used to visualize the merged time-course analysis of representative cells showing co-localization between PCV2 capsid proteins and early endosome markers (Rab5), late endosome markers (Rab7), and lysosome markers (LAMP1). Virions were stained in green, and endo-lysosomal markers were stained in red. Nuclear morphology was visualized with Hoechst 33342 (blue) counterstaining. A IF staining was conducted to visualize PCV2 co-localization with Rab5, Rab7, and LAMP1, respectively. B The percentage of cells displaying co-localization between Cap and Rab5/Rab7/LAMP1 (a–c) and the co-localization ratio indicating the proportion of PCV2⁺ pixels that also exhibited Rab5/Rab7/LAMP1 expression for each colocalized cell based on individual fluorescent pixels per cell (d–f) were quantified. Statistical differences were determined by the Student’s t-test. *P < 0.05, *** P < 0.001.