Figure 6

Effect of inhibition of endo-lysosomal acidification on PCV2 infection of T-lymphoblasts in Landrace and Piétrain pigs. A Cell viability of T-lymphoblasts was detected by a CCK-8 assay kit under CQ treatment at different concentrations. B Visualization of PCV2 infection in T-lymphoblasts after CQ treatment. C Percentage of cells displaying PCV2 infection. Cells were pre-treated with CQ (0, 1, 10, 25 and 50 μM) prior to PCV2 incubation, and the cells were cultured in the presence of CQ until 24 h. The percentage of PCV2⁺ cells (stained in green) was quantitated. All data were obtained from three pigs of each breed, and within each replicate, the mean was calculated based on ten randomly selected fields. Data represent means ± SD of triplicated assays. Statistical differences were determined by the Student’s t-test. *P < 0.05.