Figure 5

Phosphacan expression on T-lymphoblasts and its co-localization with PCV2 virions. All data were obtained from three pigs of each breed, and within each replicate, the mean was calculated based on ten randomly selected fields. A Visualization of phosphacan expression on T-lymphoblasts from Landrace and Piétrain pigs. B The percentage of T-lymphoblasts expressing phosphacan (a) and the mean fluorescing area of phosphacan per phosphacan⁺ cell (b) were quantitated. C Confocal microscopy was used to visualize the co-localization between phosphacan and PCV2. Phosphacan and PCV2 virions were stained green and red, respectively. D Co-localization assays between phosphacan and PCV2 particles were conducted, including examination of the percentage of PCV2⁺ cells (a), quantification of the fluorescing area of attached PCV2 particles per PCV2⁺ cell using ImageJ (b), determination of the percentage of cells displaying co-localization between phosphacan and PCV2 particles (c), and evaluation of the proportion of PCV2⁺ pixels that are also exhibited phosphacan expression for each PCV2-phosphacan colocalized cell based on individual fluorescent pixels per cell (d). Data are represented as means ± SD from three replicates. Statistical differences were determined by the Student’s t-test. *P < 0.05.