Figure 2

PCV2 binding and internalization into T-lymphoblasts from Landrace and Piétrain pigs. Cells were inoculated with six strains of PCV2 particles for 1 h at 37 ℃, followed by IF staining to determine viral binding and internalization. PCV2 particles were stained green, and T-lymphoblasts were stained red. All data were obtained from three pigs of each breed for T-lymphoblasts. Within each replicate, the mean was calculated based on ten randomly selected fields. A Confocal microscopy was applied to visualize PCV2 particles bound to and internalized into T-lymphoblasts. Some green virus particles were sticking to the cell membrane, while others were located inside the red cell rings and were regarded as internalized particles (shadowed bars). B The percentage of T-lymphoblasts with bound and internalized virus was quantitated (a). The fluorescing area of total bound and internalized virus particles (shadowed bars) per PCV2⁺ cell was quantitated as digital pixels with ImageJ software (b). Data represent the means ± SD of three replicates.