Figure 1

PCV2 replication kinetics in T-lymphoblasts from Landrace and Piétrain pigs. Cells were inoculated with six PCV2 strains [1121 (PCV2a), Stoon-1010 (PCV2a), 1147 (PCV2b), 09V448 (PCV2d-1), DE222-13 (PCV2d-2) and 19V245 (PCV2d-2)] at a MOI of 0.05 for 1 h at 37 °C. The data represent the mean values from three independent replicates (three pigs of each breed). Within each replicate, the mean was calculated based on ten randomly selected fields. A Representative confocal images showing strain DE222-13 replication in Landrace T-lymphoblasts. B The expression of Rep and Cap proteins was analyzed by IF staining to quantify the percentage of Rep⁺ cells (a, b) and Cap⁺ cells (c, d). The titer was determined via viral titration in PK-15 cells (e, f). Data represent the means ± SD of three replicates. Statistical difference was demonstrated between 1 and 72 h for each strain by the Student’s t-test post-Welch’s correction. *P < 0.05, **P < 0.01, ***P < 0.001.