Table 2 Details on qPCR and PCR primers
Taxon | Target gene | Primer | Primer sequence | PCR product size (bp) | Reference |
|---|---|---|---|---|---|
(q)PCR assays | |||||
 H. suis | UreAB | BFHsuis_F1 | FW (5'-AAA ACA MAG GCG ATC GCC CTG TA-3')* | 150 | [7] |
BFHsuis_R1 | RV (5'-TTT CTT CGC CAG GTT CAA AGC G-3') | ||||
 F. gastrosuis | gyrB | GB_2F | FW (5'-GAA GAC AAC CCA GCT GTA ACA-3') | 142 | [8] |
GB_2R | RV (5'-CAG CTA ATT TCC CAG GAA GTG A-3') | ||||
PCR assay | |||||
 H. pylori | UreAB | BFHpyl_F1 | FW (5'-AAA GAG CGT GGT TTT CAT GGC G-3') | 217 | [13] |
BFHpyl_R1 | RV (5'-GGG TTT TAC CGC CAC CGA ATT TAA-3') | ||||
 H. pylori | glmM (UreC) | Hpy3F | FW (5'-TTATCGGTAAAGACACCAGAAA-3') | 144 | [14] |
Hpy3R | RV (5'-ATCACAGCGCATGTCTTC-3') | ||||