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Table 1 Antibodies used in the study. 

From: Temperature impacts the bovine ex vivo immune response towards Mycoplasmopsis bovis

Antibody

Source

Identifier

Anti-CD14 (Clone CAM36A; mouse Ig1; 1:800 dilution)

Kingfisher Biotech

Cat# WS0565B-100

Anti-CD25 (Clone LCTB2A; mouse IgG3; 1:200 dilution)

Kingfisher Biotech

Cat# WS0597B-100

Anti-CD11c (Clone BAQ153A; mouse IgM; 1:40 dilution)

Kingfisher Biotech

Cat# WS0519B-100

Anti-CD16 (Clone KD1; mouse, IgG2a; 1:100 dilution)

Bio-Rad

Cat# MCA5665GA

Anti-CD172a (Clone CC149; mouse IgG2b; 1:200 dilution)

Bio-Rad

Cat# MCA2041GA

Anti-CD62L (Clone CC32; mouse IgG1; 1:320 dilution)

Bio-Rad

Cat# MCA1649G

Anti-WC1 (Clone CC15; mouse IgG2a; 1:800 dilution)

Bio-Rad

Cat# MCA838

Anti-CD21 (Clone CC51; mouse IgG2b; 1:800 dilution)

Bio-Rad

Cat# MCA5953

Anti-CD4-AF700 (Clone CC8; mouse IgG2a; 1:200 dilution)

Bio-Rad

Cat# MCA1653A700

Anti-CD13 (Clone CC81; mouse IgG1; 1:100 dilution)

Bio-Rad

Cat# MCA2338GA

Anti-MHC-II-PE (Clone IL-A21; mouse IgG2a; 1:40 dilution)

Bio-Rad

Cat# MCA2445PE

Anti-CD3 (Clone MM1A; mouse IgG1; 1:300 dilution)

Bio-Rad

Cat# MCA6080

Anti-CD8-Pacific Blue (Clone CC63; mouse IgG2a; 1:20 dilution)

Bio-Rad

Cat# MCA837PB

Anti-CD44-FITC (Clone IL-A118; mouse IgG1; 1:40 dilution)

Bio-Rad

Cat# MCA2433F

Anti-CD335 (Clone AKS1; mouse IgG1; 1:100 dilution)

Bio-Rad

Cat# MCA2365GA

Anti-TNF-α-AF488 (Clone CC327; mouse IgG2b; 1:80 dilution)

Bio-Rad

Cat# MCA2334A488

Anti-IFN-γ-A647 (Clone CC302; mouse IgG1; 1:160 dilution)

Bio-Rad

Cat# MCA1783A647

Anti-IL-4-PE (Clone CC303; mouse IgG2a; 1:20 dilution)

Bio-Rad

Cat# MCA1820PE

Anti-CD20 (Clone MEM-97; mouse IgG1; 1:100 dilution)

TermoFisher

Cat# MA1-19008

Anti-CCR7-Super Bright 645 (Clone 3D12; Rat IgG2a; 1:80 dilution)

TermoFisher

Cat# 64–1979-42

Anti-IL-17A-APC-ef780 (Clone Ebio64DEC17; mouse IgG1; 1:160 dilution)

TermoFisher

Cat# 47–7179-42

Anti-mouse IgG2a-Super Bright 436 (Clone m2a-15F8; rat IgG1; 1:20 dilution)

TermoFisher

Cat# 62–4210-82

Anti-mouse IgG2a-Super Bright 780 (Clone m2a-15F8; rat IgG1; 1:40 dilution)

TermoFisher

Cat# 78–4210-82

Anti-mouse IgG3-PE-Cy5 (Goat IgG; 1:200 dilution)

SouthernBiotech

Cat# 1100–13

Anti-mouse IgG2b-APC-Cy7 (Goat IgG; 1:400 dilution)

SouthernBiotech

Cat# 1090–19

Anti-mouse IgG2b-PE-Cy7 (Goat IgG; 1:250 dilution)

SouthernBiotech

Cat# 1090–17

Anti-mouse IgG1-PE-Cy7 (Clone RMG1-1; rat IgG; 1:640 dilution)

BioLegend

Cat# 406614

Anti-mouse IgG1-Brilliant Violet 421 (Clone RMG1-1; rat IgG; 1:80 dilution)

BioLegend

Cat# 406616

Anti-mouse IgM-BV786 (Clone R6-60.2; rat IgG2a; 1:100 dilution)

BD Bioscience

Cat# 564028

Others

Source

Identifier

Zenonâ„¢ Mouse IgG1 Labeling Kit, AF647

TermoFisher

Cat# Z25008

AF532 Antibody Labeling Kit

TermoFisher

Cat# A20182

Zenonâ„¢ Mouse IgG1 Labeling Kit, AF700

TermoFisher

Cat# Z25011

LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit, for 405 nm

TermoFisher

Cat# L34968

Brefeldin A Solution (1000X)

TermoFisher

Cat# 00–4506-51