Figure 9

High fever reduces the secretion of pro-inflammatory cytokines by primary blood cells in response to Mycoplasmopsis bovis. PBMCs from 8 individual animals were either for 18 h unstimulated (reference points for the assay), stimulated with live M. bovis at 38 °C (normal bovine body temperature, “No fever”), 39.5 °C ("Low fever") or 41 °C (“High fever”). A Cytokine/chemokine secretion in supernatants of PBMC cultures, using Multiplex immunoassay. A single measurement was done per samples tested, and each symbol represents an individual cow. Heat map shows log₂-fold changes in concentration of 15 cytokines/chemokines. For a given cytokine/chemokine, normalization was as follow: [concentration for a given animal] / [average concentration of reference points at 38 °C (“No fever”, ∅) for 8 animals]. B As in (A), but displaying the ratios: [Low: No fever]. C As in (A), but displaying the ratios: [High: No fever]. D As in (A), but displaying the ratios: [High: Low fever].