Figure 8

Influence of fever on primary blood cell stimulation by Mycoplasmopsis bovis. PBMCs from 8 individual animals were either for 18 h unstimulated (reference points for the assay), stimulated with live M. bovis at 38 °C (normal bovine body temperature, “No fever”), 39.5 °C (“Low fever”) or 41 °C (“High fever”). The fold changes analysis of activation / maturation markers was determined by FCM with FlowJo. Cells from the individual cows are represented by separate symbols; for stimulated samples, mean fluorescence intensity (MFI) values are normalized to that obtained with the reference point from the same animal. A Results obtained for the “Innate immunity” cell subsets. B Results obtained for the “Bridge innate/adaptive immunity” cell subsets. C Results obtained for the “Adaptive immunity” cell subsets. Blue backgrounds indicate a significantly attenuated activation / maturation marker expression under high fever temperature, compared to Low/No fever temperature; red backgrounds indicate the opposite. Experimental conditions were compared using one-way ANOVA followed by Tukey’s post hoc test. Stars indicate significance levels. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns not significant.