Figure 7

Induction of cytokines by primary blood cells following exposure to Mycoplasmopsis bovis under physiological temperature. PBMCs from 8 individual animals were either for 18 h unstimulated or stimulated with live M. bovis. Stimulations were run in parallel at 37 °C (standard cell culture condition, “Hypothermia”) or at 38–38.5 °C (“Body Temp”). A Cytokine/chemokine secretion in supernatants of PBMC cultures, using Multiplex immunoassay. A single measurement was done per samples tested, and each symbol represents an individual cow. Heat map shows log₂-fold changes in concentration of 15 Cytokines/chemokines. For a given cytokine/chemokine, normalization was as follow: [concentration for a given animal] / [average concentration of reference points at 37 °C (“Hypothermia”, ∅) for 8 animals]. B As in (A), but displaying the ratios: [Body Temp: Hypothermia]. C As in (B), but showing average instead of individual values.