Figure 6

Influence of physiological temperature on primary blood cell stimulation by Mycoplasmopsis bovis. PBMCs from 8 individual animals were either for 18 h unstimulated (reference points for the assay) or stimulated with live M. bovis. Stimulations were run in parallel at 37 °C (standard cell culture condition, “Hypothermia”) or at 38–38.5 °C (physiological temperature of cow, “Body Temp”). The fold change analysis of activation/maturation markers was determined by FCM with FlowJo. Cells from the individual cows are represented by separate symbols; for stimulated samples, MFI values are normalized to that obtained with the reference point from the same animal. A Results obtained for the “Innate immunity” cell subsets. B Results obtained for the “Bridge innate/adaptive immunity” cell subsets. C Results obtained for the “Adaptive immunity” cell subsets. Red arrows indicate a further increase of activation/maturation marker expression induced by 38–38.5 °C compared to 37 °C; blue arrows indicate the opposite. Experimental conditions were compared using paired t tests. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns: not significant.