Figure 4
From: Temperature impacts the bovine ex vivo immune response towards Mycoplasmopsis bovis
Ex vivo response to live Mycoplasmopsis bovis. PBMCs from 8 individual animals were either for 18 h unstimulated (reference points for the assay) or stimulated with M. bovis (live or heat-inactivated). Stimulations were run at 37 °C. The fold change analysis of activation/maturation markers was determined by FCM with FlowJo. Cells from the individual cows are represented by separate symbols; for stimulated samples, MFI values are normalized to that obtained with the reference point from the same animal. A Results obtained for the “Innate immunity” cell subsets. B Results obtained for the “Bridge innate/adaptive immunity” cell subsets. C Results obtained for the “Adaptive immunity” cell subsets. The grey zones correspond to the cells from comparators receiving heat-inactivated M. bovis, as negative controls. Experimental conditions were compared using paired t tests. Stars indicate significance levels. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns: not significant.