Figure 3From: Temperature impacts the bovine ex vivo immune response towards Mycoplasmopsis bovisInduction of cytokines by primary blood cells following exposure to synthetic TLR ligands. PBMCs from 8 individual animals were either let for 18 h unstimulated (reference points for the assay), stimulated with TLR2/4 (“bacterial infection”) or stimulated with TLR3/7/8 (“viral infection”). A Cytokine/chemokine secretion in supernatants of PBMC cultures, using Multiplex immunoassay. A single measurement was done per samples tested, and each symbol represents an individual cow. Heat map shows log2-fold changes in concentration of 15 cytokines/chemokines. For a given cytokine / chemokine, normalization was as follow: [concentration for a given animal] / [average concentration of reference points (∅) for 8 animals]. B As in (A), but displaying the ratios: [TLR2/4: ∅] and [TLR3/7/8: ∅]. C As in (A), but displaying the ratio: [TLR3/7/8: TLR2/4].Back to article page