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Figure 1 | Veterinary Research

Figure 1

From: Use of live attenuated recombinant Newcastle disease virus carrying avian paramyxovirus 2 HN and F protein genes to enhance immune responses against species A rotavirus VP6 protein

Figure 1

Construction of recombinant NDVs expressing the VP6 gene of BRV. A Schema of constructed recombinant NDV genomes inserted with the BRV VP6 or VP4 gene. B Immunostaining of HEp-2 cells infected with each recombinant NDV. The infected cells were stained using anti-NDV antiserum (green), BRV antibody (red), and Hoechst 33342 (blue; nuclei). Scale bar, 50 µm. C Growth kinetics of recombinant viruses in different cells. The cells were infected at MOI of 0.1 for each of the viruses. The copy number from the culture supernatants was determined using real-time PCR at 24-h intervals.

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Veterinary Research

ISSN: 1297-9716