Figure 5

Concentrations of IL-1β (A) and TNF-α (B) in in vitro infected porcine blood samples. The concentrations of these proinflammatory cytokines were measured by ELISA 2 h after in vitro infection of blood samples drawn from 8-week-old piglets (n = 8) with the indicated S. suis strains of serotypes 2, 14, and 9. The control plasma samples were collected prior to in vitro infection with S. suis strains (pre inf). Horizontal lines and error bars represent mean values and SDs, respectively. The limit of detection was 0.008 ng/mL for TNF-α and IL-1β and all pre-inf samples lay below that limit. The concentrations were analyzed by using Friedman followed by Dunn’s multiple comparisons test and ANOVAs with Turkey’s multiple comparisons tests, respectively. Differences that are not indicated are not significant. Significant differences are indicated (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001). Brackets (}) refer to differences to each column below the bracket.