Figure 2

Characterisation of equine enteroid monolayers. A Expression of cell lineage markers SOX9, LYZ, PCNA, EPCAM, CGA, MUC2 and DCLK1 in enteroid monolayers after 5–6 days culture on transwell supports. The gene expression was normalized to the geometric mean for the reference genes HPRT, SDHA and GAPDH and is presented as 2^−ΔCt The results are generated from monolayers originating from two horses, each used in two separate experiments, giving a sample size of N = 4. B Representative confocal image of transwell-grown enteroid monolayers stained for nuclei (DAPI) and F-actin (phalloidin). C Monolayer cross-sections stained with HE to visualize monolayer structure. D Monolayer cross-sections stained with AB to detect the presence of acidic mucins and E with AB-PAS to detect neutral mucins. Mucus-containing cells are indicated by arrows. Scale bars = 50 µm.