Figure 5

Effects of rTs-gal on the expression of inflammatory mediators. A Western blotting analysis of TLR2, TLR4, MyD88, and NF-κB expression. B Relative protein abundance was determined by densitometric analysis according to the Western blotting results, and β-actin was used as a reference. C, D, E ELISA analysis of cytokine levels (IL-1β, IL-6, TNF-α). Significant differences were indicated by P < 0.05 (*), P < 0.01 (**), and P < 0.001 (***).