Figure 7

Loss of function of TgOTU7 affects Toxoplasma gondii biogenesis and genomic transcription of the apicoplast. A Abnormal apicoplast in TgOTU7-KO mice. Parasites were stained with rabbit anti-CPN60 (green), mouse anti-IMC1 (red) and DAPI (blue) for immunofluorescence. Some daughter cells of TgOTU7-KO plants lost apicoplasts during division. B Quantification of abnormal apicoplasts in TgOTU7-KO mice, as shown in Panel A. C and D Quantification of mRNA (C) and genomic DNA (D) via qRT‒PCR are shown for the rps3 and Tuf genes. The data are from three independent experiments performed in triplicate. E Relative mRNA levels of the nuclear genes whose protein products targeted the apicoplast were quantified by qRT‒PCR. Statistical analysis was performed using the 2^−ΔΔCT method in Excel and two-way ANOVA or unpaired t test. P values: * ≤ 0.05, ** ≤ 0.01, *** ≤ 0.001, **** ≤ 0.0001.