Figure 6

TgOTU7-KO tachyzoites exhibit defects in plaque formation, host cell invasion and replication. A Plaques formed from HFFs by the RHΔku80 and TgOTU7-KO parasites. Plaques are shown as clear spots on the violet background of a monolayer of HFFs stained with crystal violet. B Quantification of the plaque number (left) and area (right) of the RHΔku80 and TgOTU7-KO parasites. C Attachment and invasion of RHΔku80 and TgOTU7-KO parasites were quantified as the ratio of attached or invaded parasites to the host nucleus. D Quantification of the invasion efficiency of RHΔku80 and TgOTU7-KO parasites, which was quantified as the ratio of invaded parasites to total parasites. E Replication of RHΔku80 and TgOTU7-KO parasites. The quantification of parasites per parasitophorous vacuole is shown. F Induced egress of RHΔku80 and TgOTU7-KO parasites. All the data were collected from three independent experiments. Statistical analysis was performed with an unpaired t test, P values: * ≤ 0.05, ** ≤ 0.01, **** ≤ 0.0001.