Figure 4
TgOTU7 expression dynamics and localization among different cell cycle stages. Parasites were stained with rabbit anti-HA (green, indicating TgOTU7), mouse anti-CPN60 (red, an apicoplast luminal protein), rabbit anti-IMC1 (fuchsia, inner membrane complex 1), or DAPI (blue, nucleus). The G1, S, early (E), intermediate (I), and late (L) stages of cytokinesis were defined by the size, morphology and DNA intensity of the cell nucleus integrated with the extent of IMC1 labelling of the daughter scaffolds and CPN60 labelling of the apicoplast.