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Figure 1 | Veterinary Research

Figure 1

From: An apicoplast-localized deubiquitinase contributes to the cell growth and apicoplast homeostasis of Toxoplasma gondii

Figure 1

TgOTUB1 is not specifically localized at the apicoplast. A Schematic representation of the CRISPR/Cas9 strategy used to construct the endogenous TgOTUB1-3HA-tagged tachyzoites. B PCR analysis of the 3 × HA-tag insertion at the 3′ end of the TgOTUB1 gene. PCR targets are shown in Panel A. C The TgOTUB1-3HA protein was detected via Western blotting using rabbit anti-HA antibodies. The expected size of the TgOTUB1-3HA proteins is highlighted by a red star. D Immunofluorescence staining revealed the intracellular localization of endogenously tagged TgOTUB1. Parasites were probed with rabbit anti-HA (green) and mouse anti-IMC1 (red, inner membrane complex 1). IMC1 was used to outline the tachyzoites, and DAPI (blue) was used as a nuclear stain.

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Veterinary Research

ISSN: 1297-9716