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Table 1 Primers used in this study

From: Characteristics of the a sequence of the duck Plague virus genome and specific cleavage of the viral genome based on the a sequence

Primers

Primer sequence (5′–3′)

Function

pUC118-CTJ1-F/R

F:TATGACCATGATTACGAATTCGCCCAATTTCCCCAGTGTTATCCTGTG

R:CAGGTCGACTCTAGAGGATCCTCTGAGGGAGGGAAGAAGAGT

To amplify the CTJ sequence for analysis of the progeny genome DR motif

pUC118-CTJs-F/R

F:ATGACCATGATTACGAATTCTGGTGTATTGCCAATCCTTTGCCGA

R:AGGTCGACTCTAGAGGATCCGGGCGTGAAATACAAACTTGAGTTT

To construct the standard pUC118-CTJ for TaqMan dual quantification PCR

pUC118-UL31 s-F/R

F:TATGACCATGATTACGAATTCATTCAATTTCGCTCGTCTCA

R:CAGGTCGACTCTAGAGGATCCCTTGTACCCAGGGATGCCAC

To construct the standard pUC118-UL31 for TaqMan dual quantification PCR

pTerS-F/R

F:CAATCCAGATGCATAGTCCGCGTG

R:GAGACGGGACTCCGAAGGTAGT

To amplify S-terminus probe for southern blot

Qpcr-CTJ-F/R

F:GGTGGAGTTGGCATGTTG

R:CTTCCATAGCAGTGCATTGA

To amplify CTJ sequence for TaqMan dual quantification PCR

Qpcr-UL31-F/R

F:CCATGAGAGCCAGATCTTC

R:CTCCCGTACTATGGCTAAC

To amplify UL31 gene for TaqMan dual quantification PCR

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Veterinary Research

ISSN: 1297-9716