Protective efficacy of multiepitope vaccines constructed from common antigens of Eimeria species in chickens

Table 2 Primers for gene amplification of four common antigens.

Source	Gene	Fragment	Primer	Restriction enzyme	Sequence (5ʹ to 3ʹ)	Accession No
E. acervulina	14–3-3	14	Forward (no ATG)	KpnI	CGGGGTACCAACAAAGCACTTGCAGCTAGCT	XM_013394831.1
			Forward (with ATG)	KpnI	CGGGGTACCATGAACAAAGCACTTGCAGCTAGCT
			Reverse	HindIII	CCCAAGCTTGTCGCGCAACAGCTGCA
	GAPDH	G	Forward	NotI	ATTTGCGGCCGCTCACGGCAAATTCCCTGG	XM_013395851.1
	GAPDH	G	Reverse	BamHI	CGCGGATCCGTTAAGAGAAGGAATAACTTTCCCT	XM_013395851.1
	Transhydrogenase	T	Forward	BamHI	CGCGGATCCAGATTGGCTGTTGGTGTTCT	XM_013397304.1
			Reverse (no TAA)	XhoI	CCGCTCGAGGCGGGTTTCAATGCGT
			Reverse (with TAA)	XhoI	CCGCTCGAGTAAGCGGGTTTCAATGCGT
E. maxima	EF-2	E	Forward	HindIII	CCCAAGCTTTTCGGTCGTGTGTTCTCTG	XM_013477319.1
E. maxima	EF-2	E	Reverse	NotI	ATTTGCGGCCGCCATGGACGAGGGAGCG	XM_013477319.1

The restriction enzyme sites are underlined
These primers have been confirmed to serve as cDNA primers for their corresponding genes. Primers with the initiator codon ATG and stop codon TAA were used in the construction of the eukaryotic expression plasmid pVAX1-14EGT, while primers without the initiator codon ATG and stop codon TAA were used in the construction of the prokaryotic expression plasmid pET-32a-14EGT.

ISSN: 1297-9716