Figure 4
From: Mitochondrial dysfunction induced by bedaquiline as an anti-Toxoplasma alternative
Mitochondrial inner membrane defects due to BDQ treatment. A Diagrams showing the different morphologies of T. gondii mitochondria. Mitochondrial morphology of intracellular parasites was observed by IFA. FLAG-labeled F1β B and C, ASAP16 D and APAP7 E parasite strains were treated with DMSO and 10 μM BDQ for 20 h and then observed after IFA using mouse anti-Flag and rabbit anti-TgIMC1 antibodies. Parasite shapes were observed with anti-TgIMC1 (green) and nuclear DNA was stained with Hoechst (blue). Scale bar = 5 μm. C, F, G Quantification of different types of mitochondrial morphology after 20 h of BDQ treatment using F1β- FLAG C, ASAP16-FLAG F and ASAP7-FLAG G strains. Quantification was performed based on 100 randomly selected tachyzoites from three independent experiments. Student t-test was used for statistical analysis (****P < 0.0001).