Figure 2

BDQ rapidly kills T. gondii tachyzoites. T. gondii tachyzoites expression firefly luciferase (TgRH-Luc) in HFF were exposed to 10 μM BDQ for 2, 4, 8, 24, 48, 72, 96 and 120 h, respectively. Then, the drugs were removed and parasites were allowed to recover for 3 A or 5 days B. The parasite numbers were presented by relative luminescence units (RLU). The experiments were performed with three independent replicates. C Parasite survival determined by immunofluorescent assay. Intracellular tachyzoites treated with BDQ or vehicle for 5 days, then the monolayers were crushed and the whole fragments were inoculated to new monolayers. The new monolayers were washed 5 h after inoculation and incubated for another 7 days. Then, parasites were fixed and stained by rabbit anti-GAP45 antibody and the nucleus was stained with Hoechst.